WG4: Digestive lipases and lipid digestion

WG4: Digestive lipases and lipid digestion

WG4 leaders

▶️ WG4 leaders:

 

✒️ Dr Marion LetisseTeam DO-IT : "Diet and food matrix in Obesity and metabolic diseases: role of Intestinal tract and innovative Therapeutics", Laboratoire CarMeNINSERM - Université Claude Bernard Lyon 1 - INRAE Hospices Civils Lyon, Hôpital Lyon Sud, Pierre-Bénite - France / Email: marion.letisse@insa-lyon.fr

✒️ Dr Leslie Couëdelo / ITERG, Nutrition Lifesciences, Université Bordeaux 1, Canejan - France / Email: l.couedelo@iterg.com

✒️ Dr Frédéric Carrière / Directeur de recherche, UMR7281 Bioénergétique et Ingénierie des Protéines, Research group on Enzymology in a complex mediumCNRS, Marseille - France / Email: carriere@imm.cnrs.fr 

 

 

▶️ Main objectives:

  • The goal of this working group is to investigate, develop, harmonize and validate methods to study lipases activity and lipid digestion in different matrices, from simple lipid emulsions to complex food mixtures.

 

▶️ Main activites:

 

WG4 Illustration A
An Example of HPTLC plate issued from gastric and/or pancreatic lipolysis
  • Obtain and test reliable sources of lipases for the gastric and intestinal phases of digestion (gastric lipase; pancreatin)
  • Optimize the activity measurement assays for gastric and pancreatic lipases, for quality control of enzyme sources and standardisation of in vitro digestion protocols
  • Find appropriate lipase inhibitor(s) for each phase of digestion in order to stop lipid digestion
  • Considering the digestion of all dietary lipids (tri-, di- and mono-acylglycerols, phospholipids, galactolipids, vitamin and cholesterol esters) with characterization /implementation of relevant enzymes such as pancreatic phospholipase A2, Pancreatic carboxylic ester hydrolase/bile-salt-stimulted lipase (CEH/BSSL), pancreatic lipase-related protein (PLRP2)
  • Propose, test and compare quantitative analysis of dietary lipids and their lipolysis products by various techniques including thin-layer chromatography, GC, HPLC, NMR, IR spectroscopy,…
  • Organise ring trials among Infogest WG4 members for validation of protocols and enzyme sources
  • Update Infogest in vitro digestion protocols based on the data obtained
  • Welcome any request from Infogest members regarding lipid digestion and give advices
  • Working group meetings : 11 labs involved from Europe
  • Publish method validations
WG4 Illustration B
pH-stat apparatus

 

▶️ Publications:

Myriam Grundy, Evan Abrahamse, Annette Almgren, Marie Alminger, Ana Andres, Renata M. C. Ariëns, Shanna Bastiaan-Net, Claire Bourlieu-Lacanal, André Brodkorb, Maria R. Bronze, Irene Comi, Leslie Couëdelo, Didier Dupont, Annie Durand, Sedef N. El, Tara Grauwet, Christine Heerup, Ana Heredia, Marcos R. Infantes Garcia, Christian Jungnickel, Ilona E. Kłosowska-Chomiczewska, Marion Létisse, Adam Macierzanka, Alan R. Mackie, David J. McClements, Olivia Menard, Anne Meynier, Marie-Caroline Michalski, Ana-Isabel Mulet-Cabero, Anette Mullertz, Francina M. Payeras Perelló, Irene Peinado, Mélina Robert, Sébastien Secouard, Ana T. Serra, Sandra D. Silva, Gabriel Thomassen, Cecilia Tullberg, Ingrid Undeland, Carole Vaysse, Gerd E. Vegarud, Sarah H. E. Verkempinck, Michelle Viau, Mostafa Zahir, Ruojie Zhang and  Frédéric Carrière.  

INFOGEST inter-laboratory recommendations for assaying gastric and pancreatic lipases activities prior to in vitro digestion studies.

Journal of Functional Foods (2021) 82:104497 (open access)

DOI: https://doi.org/10.1016/j.jff.2021.104497