WG1: In vitro digestion models

▶️ WG1 leaders:

✒️Prof Isidra Recio / Food Proteins Group, Institute of Food Science, Technology and Nutrition (ICTAN), Consejo Superior de Investigaciones Científicas (CSIC), Madrid - Spain / Email: i.recio@csic.es

✒️Dr Lotti Egger / Agroscope, Bern - Switzerland / Email: charlotte.egger@agroscope.admin.ch

✒️Dr Andre Brodkorb / Teagasc Food Research Centre, Cork - Ireland / Email: andre.brodkorb@teagasc.ie

▶️ Objectives:

• Standardisation, improvement, validation and sharing of in vitro digestion methods

▶️ Most important outputs :

✔️ Static Infogest method, Minekus et al. Food & Function 2014 : https://doi.org/10.1039/c3fo60702j

✔️ Static Infogest 2.0 method, Brodkorb et al. Nature Protocols 2019: https://doi.org/10.1038/s41596-018-0119-1

✔️ Semi-dynamic Infogest method, Mulet-Cabero et al. Food & Function 2019:  https://doi.org/10.1039/c9fo01293a

✔️ Multi-Centre peptidomics investigation of food digesta: https://doi.org/10.1016/j.foodres.2023.112887

✔️ Current advances for in vitro protein digestibility: https://doi.org/10.3389/fnut.2024.1404538

✔️ Infogest YouTube Channel @fooddigestion

✔️ Administration of LinkedIn group @Infogest

✔️ Organisation of training schools for the different protocols

✔️ Organisation of ring-trials for the different protocols

▶️ What’s next ?

➡️  Semi-dynamic IVD protocols:

• A consensus protocol for infant semi-dynamic digestion is currently established within members of WG1.
• Parameters differentiating between pre-term-, full-term infants, and children is planned.

Fig.1: Published and planned consensus semi-dynamic IVD methods for specific age groups

➡️  Monthly Viva Webinars

 

➡️  ISO 24167|IDF 261 standard method on in vitro protein digestibility

• ISO/CD 24167|IDF 261 Milk and milk products — In vitro digestion protocol for the determination of protein digestibility and in vitro digestible indispensable amino acid score (DIAAS). Draft in consultation
• A joint manuscript including all participants is in preparation.